Journal: Signal Transduction and Targeted Therapy

Article Title: The β-catenin/YAP signaling axis is a key regulator of melanoma-associated fibroblasts

doi: 10.1038/s41392-019-0100-7

Figure Lengend Snippet: SK-MEL-24; GFP/Fb spheroid and SK-MEL-24; bcat-GFP/Fb spheroid cultures were grown for 96 h. Representative bright-field images of SK-MEL-24; GFP/Fb spheroid a – d and SK-MEL-24; bcat-GFP/Fb spheroid cultures e – h were taken using a Carl Zeiss Axiovert 100 TV inverted microscope at ×5 magnification at 24, 48, 72, and 96 h. i Ki67 immunofluorescence staining (red) of SK-MEL-24; GFP/Fb and SK-MEL-24; bcat-GFP/Fb spheroids embedded in paraffin. The nuclei were stained with DAPI (blue). Scale bar: 200 μm. j Quantification of Ki67-positive cells per square millimeter of SK-MEL-24; GFP/Fb spheroid sections and SK-MEL-24; bcat-GFP/Fb spheroid sections. A minimum of ten randomly selected fields was counted for each spheroid type. k Flow cytometry was used to analyze apoptosis and death of SK-MEL-24 melanoma cells in both SK-MEL-24; GFP/Fb spheroids and SK-MEL-24; bcat-GFP/Fb spheroids using annexin V and PI staining. l Percentages of SK-MEL-24 cell subpopulations based on PI and annexin V staining in SK-MEL-24; GFP/Fb spheroids and SK-MEL-24; bcat-GFP/Fb spheroids are shown. m – p α-SMA m–n and FSP-1 o – p immunostaining was performed on 5-μm-thick paraffin sections of SK-MEL-24; GFP/Fb and SK-MEL-24; bcat-GFP/Fb spheroids. Pictures were taken using a Nikon microscope at ×20 magnification. Scale bar: 200 μm. q Collagen content in SK-MEL-24; GFP/Fb spheroids and SK-MEL-24; bcat-GFP/Fb spheroids was visualized by picrosirius red staining. Pictures were taken using an inverted light microscope at ×20 magnification. r Quantification of collagen content was performed by collagen extraction and colorimetric measurement. Collagen content was normalized to total protein content for each sample. s – v Fibronectin s – t and vimentin u – v immunostaining was performed on 5-μm-thick paraffin sections of SK-MEL-24; GFP/Fb and SK-MEL-24; bcat-GFP/Fb spheroids. Pictures were taken using a Nikon microscope at ×20 magnification. Scale bar: 200 μm. w – z Photographs were taken at ×40 magnification. Scale bar: 100 μm.

Article Snippet: Picrosirius red staining was performed according to the manufacturer’s instructions (American MasterTech, Lodi, CA).

Techniques: Inverted Microscopy, Immunofluorescence, Staining, Flow Cytometry, Immunostaining, Microscopy, Light Microscopy, Extraction

Journal: Reproductive Sciences

Article Title: Cervix Stromal Cells and the Progesterone Receptor A Isoform Mediate Effects of Progesterone for Prepartum Remodeling

doi: 10.1177/1933719118820462

Figure Lengend Snippet: Top: Photomicrographs of progesterone receptor (PR) stained cells and counterstained cell nuclei (CN) in cervix sections from nonpregnant (NP), pregnant (days 15 and 18 postbreeding), and day of birth postpartum (PP) mice. Scale bar is 25 µm. Middle: Density of PR cells normalized to CN per area to account for variability in cell nuclei density due to heterogeneity of tissue morphology within and among sections in individuals, as well as within and among groups. Data are the mean ± SE (n = 5-21/group). Bottom: As inversely related to cross-linked collagen in the extracellular matrix,2 optical density of birefringence of picrosirius red-stained cervix sections cells was normalized to CN per area. Data are mean ± SE (n = 4-10/group; *P < .05 vs NP mice or **vs NP, day 15 and day 16 postbreeding groups by 1-way ANOVA). See Methods for details about staining and analyses. SE indicates standard error; ANOVA, analysis of variance.

Article Snippet: Additional sections were stained with a picrosirius red stain kit (PK-4000; Polysciences, Warrington, Pennsylvania) to assess extracellular collagen organization and counterstained with hematoxylin (SH26; Fisher Scientific, Asheville, NC) as described previously.

Techniques: Staining

Journal: PLoS ONE

Article Title: Evaluation of synthetic reticular hybrid meshes designed for intraperitoneal abdominal wall repair: Preclinical and in vitro behavior

doi: 10.1371/journal.pone.0213005

Figure Lengend Snippet: Immunolabeling for collagens type I and III and Picrosirius red staining in implants from the different study groups (100x magnification). f: mesh filaments, P: Preclude.

Article Snippet: Picrosirius red staining showed the colocalization of collagen fibers with different orientations in those fibrotic intensely vascularized and cellular areas in the Surgipro group ( ).

Techniques: Immunolabeling, Staining

Journal: PLoS ONE

Article Title: Evaluation of synthetic reticular hybrid meshes designed for intraperitoneal abdominal wall repair: Preclinical and in vitro behavior

doi: 10.1371/journal.pone.0213005

Figure Lengend Snippet: Immunohistochemical labeling for collagen type I (A, D and G, 200x magnification), collagen type III (B, E and H, 200x magnification), and Picrosirius red staining (C, F and I, 100x magnification) in adhesion tissues from animals implanted with different meshes (A, B and C, Surgipro; D, E and F, DynaMesh; G, H and I, TiMESH).

Article Snippet: Picrosirius red staining showed the colocalization of collagen fibers with different orientations in those fibrotic intensely vascularized and cellular areas in the Surgipro group ( ).

Techniques: Immunohistochemical staining, Labeling, Staining